|
KMID : 0880220080460050502
|
|
Journal of Microbiology
2008 Volume.46 No. 5 p.502 ~ p.507
|
|
|
Generation of Infectious Transcripts from Korean Strain and Mild Mottle Strain of Potato Virus X
|
|
Choi Sun-Hee
Ryu Ki-Hyun
|
|
|
Abstract
|
|
|
|
Full-length cDNAs of two different strains of Potato virus X (PVX-Kr and PVX-Mo) have been directly amplified by long template reverse transcription polymerase chain reaction (RT-PCR) using the 5’-end primer containing a SP6 or T7 RNA promoter sequence and the virus-specific 3’-end primer, and then constructed in plasmid vectors. Capped in vitro transcripts from cloned full-length cDNAs as well as those RTPCR amplicons proved to be infectious systemically on tobacco plants. Symptom expression on tobacco plants from PVX-Mo transcripts was faster and severer than that from PVX-Kr. In replication stability test of transcripts derived from PVX clones, progeny viruses showed stable replication according to sequencing through passages. This highly infectious transcript system from the full-length cDNA clones for PVX can be useful for recombinant molecules for functional analysis of viral proteins in plant-virus interaction study as well as for expression of foreign protein in planta.
|
|
|
KEYWORD
|
|
|
Potato virus X, in vitro transcription, infectious cDNA clone, plant virus, mild mottle strain, Korean strain
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|